您的瀏覽器版本已過時。我們建議您更新瀏覽器到最新版本。
宗倬章先生紀念電子顯微鏡實驗室

《 宗倬章先生紀念電子顯微鏡實驗室 》

緣起

穿透式電子顯微鏡(Transmission Electron Microscopy, TEM)具有高穿透能力及高解析度,為科學研究上最重要的工具之一。醫學院於97學年由宗倬章先生教育基金會捐款並結合理工與民生學院重點儀器設備費,設置宗倬章先生紀念電子顯微鏡實驗室,提供於臨床醫學、基礎醫學、生命科學、食品科學、材料科學與教學等相關方面服務和研究。

儀器設備

 

穿透式電子顯微鏡
Transmission electron microscopy
型號: JEOL JEM-1400

 

超薄切片機
Ultramicrotome
型號: Leica EM UC7

 

 

玻璃製刀機
型號: Leica EM KMR3

 

 

雷射掃描共軛焦分光
光譜顯微鏡

Confocal Spectral Microscope
型號: Leica TCS SP2

 

 

服務項目

組織固定、組織超薄切片、組織染色(正染色與負染色)及樣品的觀察與照相


Photo by  Transmission electron microscope

(JEOL JEM-1400, 100Kva)

  

預約使用方法

  • 每人每次以預約一個單位(2小時/單位)為原則,且連續使用不得超過二個單位。若超過預約時間30分鐘仍未上機,本中心得將此時段安排給其他需要上機者。若發現實驗出問題無法上機,請親自來取消預約或打電話委託連絡人代為取消預約。
  • 預約方式:使用者於上班時間與管理員預約使用時間(聯絡人:吳彥陞 / 電話:(02)2905-2092)

樣品製備要求

  • 樣品請置於直徑3mm之銅網上
  • 不具揮發性
  • 無強磁性
  • 不含水分
  • 無腐蝕性

相關文件下載

輔仁大學穿透式電子顯微鏡室管理暨收費辦法

輔仁大學醫學院共軛焦顯微鏡管理暨使用辦法

研究成果

    1. Ramesh Kumar, B.Stephen Inbaraj, and B.H.Chen, (21 July 2010)Surface modification of superparamagnetic iron nanoparticles with calcium salt of poly(g-glutamicacid) as coating material. Materials Research Bulletin

    2. B Stephen Inbaraj, T H Kao, T Y Tsai, C P Chiu, R Kumar and B H Chen(14 January 2011) The synthesis and characterization of poly(γ -glutamic acid)-coated magnetite nanoparticles and their effects on antibacterial activity and cytotoxicity. Nanotechnology 

    3. B.Stephen Inbaraj and B.H. Chen (June 24, 2011) Dye adsorption characteristics of magnetite nanoparticles coated with a biopolymer poly(r-glutamic acid). Bioresource Technology 

    4. Yen-Yin Tsai, Yi-Huei Huang, Ya-Li Chao, Kuang-Yu Hu, Li-Te Chin, Shiu-Huey Chou, Ai-Ling Hour, Yeong-DerYao, Chi-Shun Tu, Yao-Jen Liang, Cheng-Yuh Tsai, Hao-YuWu, Shan-Wen Tan, and Han-Min Chen. (November 2, 2011) Identification of the Nanogold Particle-Induced Endoplasmic Reticulum Stress by Omic Techniques and Systems Biology Analysis. American Chemical Society NANO.

    5. Baskaran Stephen Inbaraj and Bing-Huei Chen (9 August 2012) In vitro removal of toxic heavy metals by poly(γ-glutamic acid)-coated superparamagnetic nanoparticles. International Journal of Nanomedicine

    6. Kun-Long Hung, Su-Jane Wang, Ying-Chou Wang, Tsuey-Ru Chiang, Chia-Chuan Wang (29 October 2013) Upregulation of Presynaptic Proteins and Protein Kinases Associated with Enhanced Glutamate Release from Axonal Terminals (Synaptosomes) of the Medial Prefrontal Cortex in Rats with Neuropathic Pain. Pain

    7. C-H Lee, S-T Lin, J-J Liu, W-W Chang, J-L Hsieh and W-K Wang (23 January 2014) Salmonella induce autophagy in melanoma by the downregulation of AKT/mTOR pathway. Gene Therapy

    8. Yi Jyun Chen, Baskaran Stephen Inbaraj, Yeong Shiau Pu and Bing Huei Chen(20 March 2014) Development of lycopene micelle and lycopene chylomicron and a comparison of bioavailability. Nanotechnology

    9. Fur-Jiang Leu, Chuen-Lin Huang, Yuh-Mou Sue, Shao-Chen Lee and Chia-Chen Wang(24 September 2014) Effects of tattoo ink’s absorption spectra and particle size on cosmetic tattoo treatment efficacy using Q-switched Nd:YAG laser.Lasers in Medical Science

    10. K Suresh Babu, M Anandkumar, TY Tsai, TH Kao, B Stephen Inbaraj and BH Chen (27 November 2014) Cytotoxicity and antibacterial activity of gold-supported cerium oxide nanoparticles. International Journal of Nanomedicine

    11. Yesudoss Christu Rajan, Baskaran Stepene Inbaraj and Bing Huei Chen (21st January 2015) Synthesis and characterization of poly (r-glutamic acid)-based alumina nanoparticles with their protein adsorption efficiency and cytotoxicity towards human prostate cancer cells. The Royal Society of Chemistry

    12. Rwei-Fen S Hunag, Yi-Jun Wei, Baskaran Stephen Inbaraj and Bing-Huei Chen (8 April 2015) Inhibition of Colon cancer cell growth by nanoemulsion carrying gold nanoparticles and lycopene. International Journal of Nanomedicine

    13. Hong-Bin Chang and Bing Huei Chen (6 August 2015) Inhibition of lung cancer cells A549 and H460 by curcuminoid extracts and nanoemulsions prepared from Curcuma Ionga Linnaeus. Internation Journal of Nanomedicine

    14. Hong-Bin Chang and Bing Huei Chen (6 May 2016) Preparation of catechin extracts and nanoemulsions from green tea leaf waste and their inhibition effect on prostate cancer cell PC-3. Internation Journal of Nanomedicine

    15. Nai-Hsing Ho, Baskaran Stephene Inbaraj and Bing Huei Chen (6 May 2016) Utilization of Microemulisions from Rhinacanthus nasutus(L.) Kura to Improve Carotenoid Bioavailability. Scinetific Reports

    16. Tzu Yu Lin, Cheng Wei Lu, Su Jane Wang (13 May 2016)Luteolin protects the hippocampus against neuron impairments induced by kainic acid in rats. NeuroToxicology

    17. Tsung-Hsuan Lai, Ching-Hu Chung, Bing-Huei Chen, Chi-Feng Hung, Baskaran Stephen Inbaraj, Ming-Chieh Ma, Han-Min Chen, Chih-Jen Tsou, Pi-Hui Wu, and Wen-Bin Wu (3 April 2016) Gold Nanoparticles Compromise TNF-a Induced Endothelial Cell Adhesion Molecule Expression Through NF-kB and Protein Degradation Pathways and Reduce Neointima Formation in a Rat Carotid Balloon Injury Model. Biomedical Nanotechnology 

    18. Hung-sheng Shang, Yi-No Wo, Chun-Hou Liao, Tzong-Shi Chiueh, Yuh-Feng Lin and Han-Sun Chiang (20 July 2016) Long-term administration of ketamine induces erectile dysfunction by decreasing neuronal nitric oxide synthase on cavernous nerve and increasing corporal smooth muscle cell apoptosis in rats. Oncotarget

    19. W D Lee, Y J Liang and B H Chen (11 Nov 2016) Effects of tanshinone nanoemulsion and extract on inhibition of lung cancer cells A549. Nanotechnology

    20. H J HSU, R F Huang, T H Kao, B S Inbaraj and B H Chen (7 march 2017) Preparation of carotenoid extracts and nanoemulsions from Lycium barbarum L. and their effects on growth of HT-29 colon cancer cells. Nanotechnology

    21. Ya-Yun Wang, Ying-Hung Lin, Yi-No Wu, Yen-Lin Cheng, Yung-Chih Lin, Chiao-Yin Cheng, Han-Sun Chiang (April 6,2017) Loss of SLC9A3 decreases CFTR protein and causes obstructed azoospermia in mice. PLOS of GENETICS

實驗室地點與聯繫方式

  • 地點:輔仁大學醫學院國璽樓MD313
  • 聯絡人:吳彥陞 先生
  • 電話:(02)2905-2092
  • 送樣時間:9:00-17:00 (週一至週五)
  • 使用者請於上班時間與技術員聯絡詢問儀器使用狀況。